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GrainGenes Reference Report: PBR-122-493

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Reference	PBR-122-493
Title	Development of novel chromosome doubling strategies for wheat x maize system of wheat haploid production
Journal	Plant Breeding
Year	2003
Volume	122
Pages	493-496
Author	Sood S Dhawan R Singh K Bains NS
Abstract	Summary: Two chromosome doubling strategies were evaluated for producing wheat doubled haploids from wheat x maize crosses: (i) in vitro colchicine application to haploid embryos and (ii) colchicine treatment through postpollination tiller injections. In the in vitro approach the haploid embryos were rescued on medium containing colchicine (at concentrations of 0.2, 0.3, 0.4 and 0.5%) and moved to a colchicine-free regeneration medium 48 h later. Embryos exposed to 0.5% colchicine had 91.67% of their regenerated plants showing chromosome doubling. In the tiller injection approach, different concentrations (0.5, 0.75 and 1.0%) of colchicine solution, which also contained 2,4-D (100 ppm), were injected into the uppermost internode of crossed tillers 48 and 72 h after pollination. The chromosome doubling efficiency varied from 33 to 100%, with 1% treatment being the most effective. No chimeras of doubled/haploid sectors were observed in the case of the tiller injection treatment and all the florets showed seed set in the doubled plants. Stomatal guard cell length provided rapid, early-stage and unambiguous analysis of ploidy level on the basis of 10 guard cell observations per plant
Keyword	anther culture [ Show all 19 ]

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