GrainGenes Reference Report: PBR-123-417
Reference
PBR-123-417
Title
SCAR marker tagged to the alien leaf rust resistance gene Lr19 uniquely marking the Agropyron elongatum-derived gene Lr24 in wheat: a revision
Journal
Plant Breeding
Year
2004
Volume
123
Pages
417-420
Author
Prabhu KV Gupta SK Charpe A Koul S
Abstract
A sequence characterized amplified region (SCAR) marker tagged to an Agropyron elongatum [ Elymus elongatus ]-derived leaf rust resistance ( Lr ) gene Lr19 was validated on 18 known alien Lr genes in near-isogenic lines (NILs) in the variety 'Thatcher', along with three wheat cultivars carrying Lr24 and two carrying Lr19 . The marker was expressed only in the Lr24 lines confirming that the marker tagged the gene Lr24 . The monomorphic expression of the SCAR marker in 10 NIL pairs for Lr19 and Lr24 revealed that each NIL pair possessed the same gene, Lr24 . The donor parents used in the NIL pairs for Lr19 ('Sunstar*6/C80-1') and Lr24 ('TR380-14*7/3Ag#14') amplified the same fragment. Non-segregation for leaf rust in the F SUB 2 population of the cross between the above donor parents confirmed the presence of the same gene in the two parents. Apparently, a genuine parent stock of 'Sunstar*6/C80-1' was not involved in the development of the NIL pairs for Lr19 due to an improper maintenance breeding protocol either at source or destination which went undetected in the absence of signs of virulence for either gene in the region
Keyword
leaves resistance wheat
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PBR-123-417
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||