GrainGenes Reference Report: PCT-42-207
Reference
PCT-42-207
Title
Isolated wheat microspore culture
Journal
Plant Cell, Tissue and Organ Culture
Year
1995
Volume
42
Pages
207-213
Author
Gustafson V Baenziger P Wright M Stroup W Yen Y
Abstract
The use of doubled haploid plants in a wheat breeding program requires an efficient haploid production system While the techniques for producing doubled haploids from anther culture are well established, those for isolated microspores are complicated and inefficient Four methods of isolating microspores from anthers (blending, stirring, macerating, and floating) were compared Isolated microspores were washed and cultured in liquid medium The effects of pre-isolation mannitol conditioning, cell density, culture dilution, and sucrose centrifugation on microspore viability were evaluated Isolation by blending gave the highest initial microspore viability (75%) Mannitol conditioning and purification by sucrose centrifugation had a detrimental effect on initial viability. An initial microspore density of 2 X 10(5) microspores per ml was necessary for continued microspore viability. One hundred and nine haploid or spontaneously doubled haploid plants were regenerated from microspores isolated without mannitol conditioning using the blending method. Based on this research, blender isolation with an initial density of 2 X 10(5) microspores per ml is recommended for isolated microspore culture.
Keyword
anther-culture
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||