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GrainGenes Reference Report: PCT-43-79

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Reference	PCT-43-79
Title	Culture conditions for efficient induction of green plants from isolated microspores of barley
Journal	Plant Cell, Tissue and Organ Culture
Year	1995
Volume	43
Pages	79-81
Author	Salmenkallio-Marttila M Kurten U Kauppinen V
Abstract	Subculture regime and carbohydrate concentration of the medium had a marked effect on the regeneration of green plantlets from mechanically isolated microspores of Hordeum vulgare L cv Kymppi A sevenfold increase in the yield of green plants was obtained by shortening the suspension culture time of the developing proembryo mass from 4 to 3 weeks A further twofold increase was obtained by increasing the maltose concentration of the microspore isolation medium and of the culture medium Under optimal conditions, a mean of 169 +/- 97 green plants per spike were regenerated
Keyword	androgenesis [ Show all 12 ]

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