Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Sequence Report: CD373425

[Submit comment/correction]

Sequence	CD373425
Contig	Ta.28365.1.S1_s_at NSFT03P2_Contig18665
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC344203
wEST map position	CD373425
NCBI UniGene	Ta.28365
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001065684.1 Os11g0135400 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	CS wheat unstressed etiolated seedling shoot cDNA library
Tissue	Shoot; etiolated
Developmental Stage	Five day old seedling
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE0325_A02_A03ZT CS wheat unstressed etiolated seedling shoot cDNA library Triticum aestivum cDNA clone WHE0325_A02_A03, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0325_A02_A03
Probe	WHE0325_A02_A03
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttacacactaacaaacagcataattaattcttgt tcaaggtaataataagacttgaaaaacttcagtgttcaacacttaacaaa actggcgactggacacggctttatgaaggaaggcacacagcttagtcgtc gaacaggctgaatcccatctcgccatctgattcctcctcgggctcgtcct tcttctcctcctccttcggggcagcggcggcagcaccaccggaggcagca gcagcaggggcagcaacggcaaacttgcttgggtccttgaggtactcctt gatcttatcagcatgatcatatgagtagtctgtctccaaagcaaccgcaa gaacattcttgtatgcattgaggaacatgtgtggcgcagcagccatggtg gggtatgagatcgccagggacagtgaggcaaccatggacacaccagaagc aaacttgtccatcaggtcttcctcggtgaggtcaagaacctcagggctga agactgacccgctgtcatagacattgcagatgaccagcccataagagaag gggcggataccaagcttggcaagcagggcagactcagaggagcccacctt gtcacccttctttatcagctccacagggatagtaatttccacagtgccct tgttaatcttggtgggaatgttgag

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.