GrainGenes Sequence Report: CD373454
Sequence
CD373454
Contig
Ta.2200.1.S1_at
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC287762
wEST map position
CD373454
NCBI UniGene
Ta.2200
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001054668.1 Os05g0150800 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AS 1BS 1DS
Clone Library
CS wheat unstressed etiolated seedling shoot cDNA library
Tissue
Shoot; etiolated
Developmental Stage
Five day old seedling
Data Source
genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title
WHE0330_H05_O10ZT CS wheat unstressed etiolated seedling shoot cDNA library Triticum aestivum cDNA clone WHE0330_H05_O10, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0330_H05_O10
Probe
WHE0330_H05_O10
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttttggtgagcgactgagcgtcgtttattgatct
cgaaatagcgcaagacatgacaaaatggggagacgattttagtctaggca
ccagttacacatcatcccacatac
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CD373454
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||