GrainGenes Sequence Report: CD373531
Sequence
CD373531
Contig
Ta.1048.1.S1_at NSFT03P2_Contig17475
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC278151
wEST map position
CD373531
NCBI UniGene
Ta.1048
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wle1n.pk0057.g6:fis, full insert mRNA sequence'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL 1BL 1DL
Clone Library
CS wheat cold-stressed seedling cDNA library
Tissue
Seedling
Developmental Stage
Five-day old seedling
Data Source
genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title
WHE0370_G03_N06ZT CS wheat cold-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0370_G03_N06, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0370_G03_N06
Probe
WHE0370_G03_N06
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttacagaggaatggcaaaccaccctgtatgtgga
gcacaaggggtctgatctcgcacgtaatataatgcccacagaatcctcag
tttcgcttctgatctcgggtacgaatgctataagcacataggcaccggtg
tctgctgtttctggtactacaggttcagttcagtctggcgagacatcaaa
gccagtacattttattgcggagcacgacgacgcctcgatgccccgcagca
caaccggtgtttggaggcaacgaaagcggccgaacaaaaattgcatgagt
tcaagtgtgaaccgttcgatataggctgagcaggtcgccgcctagctctc
ctgcgcgaacttgacccccttctcgatggaagacagcagctcgcccttga
ggctctccagcccctccttctccaaggccgacagctcgcccagccccatc
acttcctccactccactcttgcccagcctcaccttggaggcaaagaatgg
cagctctgttacggttgattggacaaaagagcactctacaatgtcaggaa
caccgttgagccccttcaagcatgcatctccaaaaactgcaccagcatat
gccatcgacaatgttgctgagccctttccagccttcgcttcaacaacttc
cg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CD373531
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||