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GrainGenes Sequence Report: NSFT03P2_Contig17177

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Sequence	CD373555
Contig	Ta.28253.1.S1_at NSFT03P2_Contig17177
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC280868
wEST map position	CD373555
NCBI UniGene	Ta.54825
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001032193.1 hypothetical protein LOC641320 [Danio rerio]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	1DS 3DL 5AL 5BL 5DL
Clone Library	CS wheat etiolated seedling root cDNA library
Tissue	Root
Developmental Stage	Five day old etiolated seedling
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE0418_b09_c18zT CS wheat etiolated seedling root cDNA library Triticum aestivum cDNA clone WHE0418_b09_c18, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0418_b09_c18
Probe	WHE0418_b09_c18
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttattttttttttttttttttttttttttgantaggggac tcgcacgggaaaatcttacaaagactctggtagatagtttgacactcccc ccacgctagcaccaaactttttggcatgaaaaaaaaaaccagaacatgcg gcatatgattcagcaccggtcaaatatagcagcccactgaacatattttg acccaacaaattaatccggtaatacagtcaacagaatcccagcaatctca aacatagtagcttacatggcaaggacttgagaaaagaacagccagaatag attcagaagacccagacaactcgcaacttagaagcacttccggtggacaa tcgctggacctgactcatcatactcacccttcgagatccacatctgttgg aaggtactaagggaggcaagaatcgaccctccaatccagacactgtactt cctctcaggcggtgccaccaccttgatcttcatgctgcttggggcaaggg cagtgatctccttgctcatacggtcagcaatacccgggaacatagttgag ccaccactgagcacaatgttaccatacagatccttcctgatatccacatc acacttcatgatagagttgtaggtggtctcatggattccagcagcttcca tacc

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