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GrainGenes Sequence Report: Ta.5796.1.S1_x_at

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Sequence	CD452825
Contig	Ta.5796.1.S1_x_at Ta.5796.3.S1_a_at NSFT03P2_Contig14984
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC278231
wEST map position	CD452825
NCBI UniGene	Ta.5796
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Gamma-glutamylcysteine synthetase'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	1BS
Clone Library	CS wheat etiolated seedling root normalized cDNA library
Tissue	Root
Developmental Stage	Five day old etiolated seedling
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE1132_F10_L20ZT CS wheat etiolated seedling root normalized cDNA library Triticum aestivum cDNA clone WHE1132_F10_L20, mRNA sequence.
Strain	lab_host E. coli DH10B
Clone	WHE1132_F10_L20
Probe	WHE1132_F10_L20
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttcgtctcaaacactctgaattattatttctgggaa atcaggttccttccatcatcacgcgagatggatttattcggaatacaagc cgttcatcaggaaaagatgaaaaagggatcggtacaaacaggtgcttgtt ccggctccctggagatgcgagaaaaatcagaagctgtaaaaggtccacac agccaaagaaagaaaggcggcaagccacaaacagagtgttccgagctcaa aattattcgcagccgcgagaccagagagtaggcttccctggtcaggtggc ttgtagaattggggacgggattttgtatggggatacaatctgaagaccct gtccaccttgtccagcttccaccgaagttatatacgatatcagcatatca atataacaactcctcgaacacagggtccacgctgcgctgccacttcatct cgtatagattcagaagcttctcggcaggtgtcactcctgttctaacaact tcgtcgacttctctcaggaaaccaacctccttgtatcctcttctttctaa tccattcttagctaactggagaacatcttcagctaaatctcttacatacc catcccnggaatggcgtcttcaaaccagttactggcaccttccgtctcaa catctctctttcttcctttgtccagtcagaagtcatgtcaataatgctct gtaatgattcgtcgtcat

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