GrainGenes Sequence Report: CD453249
Sequence
CD453249
Contig
NSFT03P2_Contig11650
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC4274
wEST map position
CD453249
DB Remark
Locus Source: Secale cereale (rye)
Keyword
EST
Species
Secale cereale
Cultivar
Blanco
Chromosome
5AS 5BS 5DS
Clone Library
Rye anther cDNA library
Tissue
Anther
Developmental Stage
Adult plant before anthesis
Data Source
genbank Release 136, Jun 15 2003 genbank Downloaded 2008-2009
Title
WHE1805-1808_M11_M11ZT Rye anther cDNA library Secale cereale cDNA clone WHE1805-1808_M11_M11, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1805-1808_M11_M11
Probe
WHE1805-1808_M11_M11
Remark
DB_xref: taxon:4550 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A ) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
atttcgatctattaagtagcacaatgtggcacttaacggggggaagggct
cgatacaatagttcaagtaattttatttataacatgataagcagtgggtt
catgaagtacagaaaactttcaaggattacaaattgatcctgaccagatg
ggcaatgttagaaacttgaaggggcaggaacacatcatttgattagtcag
gtgtggcaacgccttggttaagctcagcaaatcgcatgccaaccctcatg
gagtgcttcaggaacttctcagcgcagcggcggacacacgactcctcttg
cttgtccagagtcttcctccggaatgtgtcaacacagtccttgaagcacg
tctccaccagtgcattgtacatccgtagactgtcacgcatctggagctgt
tcgatcatggcggacatgcgcatcttgtcctcgtcggggaggttgtcgag
gtcgccgagcatgctcttgtccatggttgccgccgccgcacgaagagcct
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CD453249
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||