GrainGenes Sequence Report: NSFT03P2_Contig17688
Sequence
CD453254
Contig
NSFT03P2_Contig17688
External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
CD453254
DB Remark
Locus Source: Secale cereale (rye)
Keyword
EST
Species
Secale cereale
Cultivar
Blanco
Chromosome
3AL 3DL
Clone Library
Rye anther cDNA library
Tissue
Anther
Developmental Stage
Adult plant before anthesis
Data Source
genbank Release 136, Jun 15 2003 genbank Downloaded 2008-2009
Title
WHE1808_B04_D08ZT Rye anther cDNA library Secale cereale cDNA clone WHE1808_B04_D08, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1808_B04_D08
Probe
WHE1808_B04_D08
Remark
DB_xref: taxon:4550 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A ) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttttttttttaacaagccacctttcaatt
tcaacaatgcggacactcgttaagttcttctacacaagggtaatgtgctg
ggggagatacagatcagtcagaaggtgccccagggtgaagggaaccaacc
aacacaggtgtacaaccaaccaaatagctcgagcactattcattcaaata
cttccttccaacgcaaacatctccaatttgttcttaccaacaaaacggca
ctagattgctggctaaagtatgatgggttattacatcaagggcagcatgg
ggttgtcacccaccactggcatacagacgagtccactcctttgctgtttc
aacagcttctgcctcgttggatttccagtgcttaacaatgttttcaaaaa
gagggtcatctggatttggtgcacttaggagtgcctggatactcaaaaga
actgttccaatctgaagagctggactccatttatccttgagaatgtcaag
gcatatcctaccaagcttgtcaatgttgggaaggtatatttttgtcagaa
acctaacctttggggcagccatcggatattcctcaggtaaaaagagttcc
agcttaaaaactccccctt
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig17688
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||