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GrainGenes Sequence Report: NSFT03P2_Contig16582

Sequence	CD453296
Contig	NSFT03P2_Contig16582
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC4494
wEST map position	CD453296
DB Remark	Locus Source: Secale cereale (rye)
Keyword	EST
Species	Secale cereale
Cultivar	Blanco
Chromosome	5DL
Clone Library	Rye anther cDNA library
Tissue	Anther
Developmental Stage	Adult plant before anthesis
Data Source	genbank Release 136, Jun 15 2003 genbank Downloaded 2008-2009
Title	WHE1821-1824_N01_N01ZT Rye anther cDNA library Secale cereale cDNA clone WHE1821-1824_N01_N01, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE1821-1824_N01_N01
Probe	WHE1821-1824_N01_N01
Remark	DB_xref: taxon:4550 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A ) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttttttttttttttttgaaaaaagggtgcgtttt gttcaagtacatgctaaacaagatgcctggaaccagcttccggttccatc agcctggaccaggctaaactgaccggccaactcaagatgcaggcgactgt gtaaattccaggttgccaaccaaacaccatccagacacgcagcttccagg ccaggccgatgtgccccaggagccaaacacacccaagtccgcagccaact agtaaacgtgccacggcaaagggaaacaagaaagcaaataattttcctgg acgacccggtattttgtgaagtagttatatcatatactagcaccaaaagg gtaagaaccctaacatggaaaacttgagaacaacagaaacgaattcatct gctggactgacgatcacaacatcctgcctgcctactgctgggcgcactgc acccgctggccgccaccaccaggcatgtcctcatcctcgtcgtaggcctc ctgcgcctgctgctgctgccgcctccgcatctcctcctcaatgtctatgt cgtaggccatcgtctcctcgcactcatccagctccatgtccgtgtactgc gacgccggcttgggcgggaggacactctccagcgccttgcactgctccag gctcagcgagtcggggaaatccacgtgaagtggatgtacagcttgccctt catgaac

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