GrainGenes Sequence Report: Ta.11488.1.S1_s

GrainGenes Sequence Report: Ta.11488.1.S1_s_at

Sequence

CD453367

Contig

Ta.11488.1.S1_s_at

Ta.14561.1.S1_at

NSFT03P2_Contig7405

External Databases

TIGR Gene Index

wEST map position

CD453367

NCBI UniGene

Ta.14561

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, moderately similar to NP_190723.1 FER (FERONIA); kinase/ protein kinase [Arabidopsis thaliana]'

Keyword

EST

Species

Triticum aestivum

Cultivar

TAM W101

TAM W-101

Chromosome

1BL

1DL

4BL

Clone Library

TAM W101 wheat drought stressed leaf cDNA library

Tissue

Leaf

Developmental Stage

Full tillering stage

Data Source

genbank	Release 136, Jun 15 2003
genbank	Updated Nov 2006

Title

WHE1411_H10_P19ZT TAM W101 wheat drought stressed leaf cDNA library Triticum aestivum cDNA clone WHE1411_H10_P19, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE1411_H10_P19

Probe

WHE1411_H10_P19

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 65% and 78% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close), at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttttttgcacatcactttctttttctcatacagtatt
acatgttggatcaacaacacatagcagtggtggtacaataacaacatgca
aataagcatagtcattacaacacttgaagaagaggaagaagaaaaaaaac
aaggcaagacagaaatatctaatatggaacaattttttgacttctaatgc
ataacttgcgagacagcaagagcacaaacgcatgaaaatatgctgaatga
ataaattaggcaggccccaacggtgactgactgaaaagcagacccagatt
tttacctcaccgccccttggggttcatgatctgtgagaacaccgcgctcg
gcgtcagcccatccgagtcgatgctcgcgacactttggtcacccatgctt
agggaagttgttgtggtcgtagtggtgctggaatcaattgatgggtcatt
gggatccttctttccaaccatcacgaggggagtgccctcatctgacatcc
cacacccaatgcttccactctcctccgcgctttcctgcatctgaagagca
aactccaagttccaaagcacatctcccatcgaagggcgctcgatgccatt
gtcgg


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