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GrainGenes Sequence Report: NSFT03P2_Contig8256

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Sequence	CD453431
Contig	Ta.7263.1.S1_a_at Ta.7263.3.S1_a_at NSFT03P2_Contig8256
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC293445
wEST map position	CD453431
NCBI UniGene	Ta.7263
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_173855.1 myosin-related [Arabidopsis thaliana]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	6AL
Clone Library	CS wheat heat stressed flag leaf cDNA library
Tissue	Flag leaf
Developmental Stage	Full tillering stage
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE1663-1666_N05_N05ZT CS wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1663-1666_N05_N05, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE1663-1666_N05_N05
Probe	WHE1663-1666_N05_N05
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttttttttttttttttttttccccagcgtaaaaa agaagggtattgttatcttctgcacatattacatcctgcttgcggaattt ggcacatcgtgctcgagtacaaatttgaaaagggagcaggagacaaaaag aatgacatttctatagtatataaaaaggagagagaaaaaagtaaaactat ggatccgcgctattgtacagttatgctatgagggggttggttattcacaa gcagtgtcacaagaggctgcctaccaaccataggtgcaggaagagccaat acgccaagagtcctagccttgctcgcggccggttcatcaaaatttggctc cctgagacccagatgccatcaactttgtcagcgatgtgctttccttgtgc tggaatcaagccagatgtatgcaatgacttaatacgccctttgtcatcca aatcatgtgcagcaattagtcgttcagactctgagcccatattaaaaaca agatggtcggatgaacccttccgcattactctagcatgtgcagcagtagg gacagatgaaaccgatgccttccccattggcccctcttcaatctcggata tgtctgaaccaattgccgaggaagatgcgtcaaatgctctgtcttg

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