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GrainGenes Sequence Report: CD453491

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Sequence
CD453491
Contig
Ta.2868.1.S1_at
NSFT03P2_Contig17520
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC279065
wEST map position
CD453491
NCBI UniGene
Ta.2868
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001064476.1 Os10g0377400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL
1BL
1DL
Clone Library
CS wheat heat-stressed seedling cDNA library
Tissue
Leaf
Developmental Stage
Two-week old seedling
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
WHE0764_D05_G10ZT CS wheat heat-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0764_D05_G10, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0764_D05_G10
Probe
WHE0764_D05_G10
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttttttttttgccgtagcaacgcatcagc
atatgagctagtatataatatatgtataacattacataaaggcttgatgg
tgttctgagtcatcatacacggtaattgataatcaaccccacggctcgga
cctatgaaaagggagttcatctgctcactccctaacctcatagtcagtgg
agacgagtgcaaaatcttgctctgccaccgaccccatccatctcaccaaa
tgaaccaatacttgaaacctaagactacctcagtagggatctatatccag
acagcgtgaaaaacacacacaaatccaacaaatctggtaggagagattac
atagaactgcagcacttgctctttgggatctcctgtgctgggcctgggaa
aaaaaccttcttgccggccagcgggggcgcaacaccattggcctttgggt
cggtcacaatgttcttcttgctgtgga

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