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GrainGenes Sequence Report: CD453548

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Sequence
CD453548
Contig
Ta.2429.1.S1_x_at
Ta.2429.2.S1_a_at
NSFT03P2_Contig16703
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC281440
wEST map position
CD453548
NCBI UniGene
Ta.54117
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001043653.1 Os01g0633000 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AL
3BL
3DL
Clone Library
CS wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
WHE0804_B05_D10ZT CS wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0804_B05_D10, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0804_B05_D10
Probe
WHE0804_B05_D10
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttttttaggaaaatttggttgcataaaat
caagctttttagagcaccgatttcatgaaactcaacgttgacacatactg
gaaggtagtattgctagagtcgttgcgaacattacccgaacacctcgagg
gcaatgcaaatgcaatccccgtgaaccaaatcaatcaacgtgcagacagt
acagctggggaatcaagccacgcaaactagcataattgagttcgggagtt
aacaacgagagaaacgcagcaggaagacgaggagcaggagaggcagagcc
acatcatttgcgggtctcgtcgtcctccggctcaaactccgggtcgccgt
cagggtgcatctccagccactgctccttggtccagtactccttgacgtgc
ccccacttcttgcggaacttctcgatctggctgtccatgacgaccagcgc
cgaggagtcgccgacgtagtaggggtggttccccgaccagacgtccaccg
agtactccggcttcgtgccccccgtcaccagcaccagctcaccgttgcag
tacaccttggcgtcctcgaagatctccgggtgcagccccttcttccgcat
cgagcacctcatcccctggg

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