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GrainGenes Sequence Report: Ta.7509.1.S1_at

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Sequence
CD453556
Contig
Ta.7509.1.S1_at
NSFT03P2_Contig17451
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC327631
NCBI UniGene
Ta.54911
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_172221.1 AtRABA2b (Arabidopsis Rab GTPase homolog A2b); GTP binding [Arabidopsis thaliana]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
CS wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
WHE0806_A04_A08ZT CS wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0806_A04_A08, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0806_A04_A08
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttaatacaagagagaatatatatcgcatt
gttcctgctccggtaattcggggcatcagatgtcttaggaggacaaacat
atacctgaaaggaaacatgatattgctctgaaactgtagtaagtctgact
gacaaacaatggtctctaccaaaaatttgcaactacacacggtatctacc
aaggataatttctttctagcagtatgaactttaatacaaccccaccagaa
ttacaggccaaaaggagtcaaagggacccaactaacacctagagtgccgt
gagatccatgtgagcatccttttgcagaatcaccacttctctgttcgcac
ctaggtagaacagcaccctttcttcatgttgccggatgaatcggcaacat
tgatggtggttccttgagtcggaggtccacttcctgcagcctcctgggca
gccagcgccttcttgcttatgatctgatggatgtcggaaaggatagtctg
aaaccccttctcaacgttcagtgcttccagtgccgaggtctcaaggaagg
atatcccttccttctctg

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