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GrainGenes Sequence Report: CD453559

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Sequence	CD453559
Contig	Ta.6608.1.S1_at Ta.6608.2.S1_a_at NSFT03P2_Contig15258
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC322282
wEST map position	CD453559
NCBI UniGene	Ta.6608
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001051034.1 Os03g0708000 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	4BL 4DL
Clone Library	CS wheat vernalized crown cDNA library
Tissue	Crown tissue of seedling
Developmental Stage	Five-week old seedling
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE0806_C07_E14ZT CS wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0806_C07_E14, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0806_C07_E14
Probe	WHE0806_C07_E14
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tttttttttttttttttttttttttttttttttttttagccccaaacata attcagacgacacaaattacagtactgatctataaattacccccaaaatt caaagtggctttaagcagcaaaattcactgaaatttgaacagtggtaaca acagtgtatagtactaccaattatgcgtgacatgaacacgtacgagctgc acatgatgtgtaggcaaactggcaaagacgaaccaactcatcaatcaagt aaattaaatcctgcaaagcaggttatggtaaccatgcccatatacatgtc gcaaaatccggcgccatcgtcgatcttagctgaagcaccaaccatgtcac gcccgtgcccgcgagactgtacgtgaggcgccggtgcatgcgtgacagga cacggacacacaccgtgactgacgagtgacaccaagcagaaaaggcatgc accacccacctaccgagcaagttgtttgctgcttgtcctccagctcgccg gctccggcgcgcgccgccgtggtactatgtagctagggcttgtgcaggac ccggccggcgacgacggcggcctcnatgacgacggtgatcacgtcgatga cctcgtcgatcatgcacttgttcccctcgaacgtgcccgtgccggcccgc agctccgccaggcactccagcaggccctcgttgcact

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