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GrainGenes Sequence Report: CD453614

Sequence	CD453614
Contig	Ta.787.1.S1_x_at NSFT03P2_Contig17425
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC321546
wEST map position	CD453614
NCBI UniGene	Ta.787
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001045156.1 Os01g0910900 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	6BS
Clone Library	CS wheat vernalized crown cDNA library
Tissue	Crown tissue of seedling
Developmental Stage	Five-week old seedling
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE0821-0824_A09_A09ZT CS wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0821-0824_A09_A09, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0821-0824_A09_A09
Probe	WHE0821-0824_A09_A09
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttttttactacaaatcactataactttattctgg ttcacgattagcgcttatttaagcgctgcacgggcgcgcgataggggctg atggccgccaagcacgcacgcctgcatacgctatacgactgtgcgcaaca tcacacatacatatactgctgacatgcatacatatacacacatcgtagtg tgctccgttacgtgtaagctcagtcatcggccctgacgacgaatttctcc ttgcgctcgtggaactccccggtgcgggtgttgaagtcctcctcgagcct gctctcgcgcacctcgaagtgaccagagccgcggccgtggtgaccgtggt gaccgtggcggcggccggaggtgtcnaactcctgcacgaacgtctccttg acgatgtgctcctgcacgccgccgccgcggcgggccgggttgcccaccgc cctcacctcctccgagcagtagtccacctcctggaagtgcgccatgccga tcggtcgatgttactatgtgcttggttgggtttgtgcct

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