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GrainGenes Sequence Report: Ta.23822.1.S1_at

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Sequence
CD453636
Contig
Ta.23822.1.S1_at
NSFT03P2_Contig7733
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC281905
wEST map position
CD453636
NCBI UniGene
Ta.23822
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'CYP71C8v1 mRNA for P450'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5AS
5BS
5DS
Clone Library
CS wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
WHE0825-0828_J13_J13ZT CS wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0825-0828_J13_J13, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0825-0828_J13_J13
Probe
WHE0825-0828_J13_J13
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttttttttttagggccttgcctccaaagcacata
atataagtccatcacaactcaaatacattatttttcatgactactacaac
ataactgcactataaccagtgcattaaacttctcgtccatcttatcgatc
gccagcaacaatagatggacaacatgcttattttagccctcaccttaatt
attgcgcacaagtctaaataggcttacacttgggatcgggggcactacta
gttattaggcaatccttggaacaaggtagagtttctcatttcggcgaagc
atcagcccgaacgtctcagccatactaacctttccaccagcaccatcttt
ctcattgggaacctcccagtcaaaatggtacatgaggtttacgagcataa
tctccatggtcgcgagtgcgaaatttgccccggggcaaatcctgcgtccg
gatccaaatggaagatacttaaaatcttgacccaggttgctagatttctc
ctgaccatcttgtaggaacctctcgggcatgaactcctccggtctctccc
aagcctttgggtcct

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