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GrainGenes Sequence Report: CD453641

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Sequence	CD453641
Contig	Ta.27454.2.S1_a_at NSFT03P2_Contig18709
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC361094
wEST map position	CD453641
NCBI UniGene	Ta.54796
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Cyclophilin'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	1AL 1BL
Clone Library	CS wheat vernalized crown cDNA library
Tissue	Crown tissue of seedling
Developmental Stage	Five-week old seedling
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE0825-0828_L08_L08ZT CS wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0825-0828_L08_L08, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0825-0828_L08_L08
Probe	WHE0825-0828_L08_L08
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tttttttttttttttttttttttttttttttgcagcaaaagagaactgac aacagaacggtggtgtatgataccttcaaagttgccagccagatcacggg taagcaagtcattgtacttttttatagtaaagaggaaaaaacttgccgtc taaattcacacggaagtacacgaaaacagtccggataataacgcttctac aacggaaactagtcaccgtagctttccaccagagaccgtttcaggtcatc acagcggcacttccccgctgtcagcaatgaaaactttgctctttggtgtc ccgttctgcttgccctcggcctcgaccttgtagaccacgtccattccaga caacaccttgccgaacacgacgtgcttgccatccaaccagctcgtagtta cagtggtgatgaaaaactgggatccgttggtatctctcccagcattggcc atggaaaggtaacctggtccagtgtgcttgagcttgaagttctcatcgcc gaactttgtgccgtaaattgattcaccaccccttccatcaacaagagtga agtcgcctccttggatcatgaagctggggataa

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