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GrainGenes Sequence Report: NSFT03P2_Contig18099

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Sequence	CD453731
Contig	Ta.14625.2.S1_x_at NSFT03P2_Contig18099
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC355103
wEST map position	CD453731
NCBI UniGene	Ta.14625
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to XP_001922672.1 PREDICTED: hypothetical protein, partial [Danio rerio]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	1AS 1BS 1DS
Clone Library	CS wheat 20-45 DAP spike cDNA library
Tissue	Spike and seed
Developmental Stage	Adult plant
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE0856_E06_I12ZT CS wheat 20-45 DAP spike cDNA library Triticum aestivum cDNA clone WHE0856_E06_I12, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0856_E06_I12
Probe	WHE0856_E06_I12
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 20 DAP and seeds at 30 to 45 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 20 DAP and seeds at 30 to 45 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttttagataaaactttatttgtattttattccat aatttaaactagtttgtgcgggtcacacatgacattgtgtgacactttat ttgtcaccgctacatcgacatatccatcgactaagcaacgacaatccaac tatatattactagagatattttcttatcagtaggcaccaactcgggtgcc aacgccgaatggcacaatagtggtggtgccgtacaccggcacgttgacac tgcacatcgttggcagggtacgaagtgcaatggaagtcatcacctcaagt tgagctatttggtgtggctgcaacaaggtaccctggggtacctgttgttg ttgaggccattgacccagttgttgttgttgaggttgttggaaagaacact gtccgagctgcttctgttgttgttggggttgggagacaccttggaccgac tgttggggttgttgctgctgaggttggttcaaaccctgaccatgttgttg ttcttgtaggacgatcgagtagatgatggcacggattgcatcgtagcgtg attgttcggggatttgcggcagctgccggcaacattgttgctgcatcaca tggcaactactctgccacaacatttgcgacctagcaagactttgtggcat tgccacagggctgcattgctgctggaggaatacttgcatgggtttagctg ctgca

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