GrainGenes Sequence Report: Ta.2448.1.S1_at
Sequence
CD453766
Contig
Ta.2448.1.S1_at NSFT03P2_Contig18544
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC281252
wEST map position
CD453766
NCBI UniGene
Ta.2448
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Hypothetical LOC542894'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
7AS
Clone Library
CS wheat 20-45 DAP spike cDNA library
Tissue
Spike and seed
Developmental Stage
Adult plant
Data Source
genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title
WHE0874_B11_D22ZT CS wheat 20-45 DAP spike cDNA library Triticum aestivum cDNA clone WHE0874_B11_D22, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0874_B11_D22
Probe
WHE0874_B11_D22
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 20 DAP and seeds at 30 to 45 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 20 DAP and seeds at 30 to 45 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttcaaggaacatctgttcattatttccatgttgcaattagtactag
gcacacatgatgatgcatgacattttattttgccactgctatattgatca
actagcaggtaccatcggtatagctagctgtcatggtaatgctatagggg
gtggtggtggtggctgggcattgtacagggatgtatattttgcacatcga
cggtagggtgtgaagtgacatcctcaggccctcaagttgtacttcctgtt
gtggttggtgatgttgttgttgcctgatagactctaccacattatgaatg
gcttggcagcggaattgctctgggatctgtgctagctgccggcagcattg
ggactgcacgttctggcagctggtgggcttctgcactggtgtatgcggga
atggcactgtcattggcctacattgatgttgtaggaatgtcgcacacggt
tttaactgctcctgcaactgttgttgttgttgtcgccccagttgttgctg
ttgctggcattgtaccgggacgtagatgttgcacatagacggcagcct
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: Ta.2448.1.S1_at
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||