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GrainGenes Sequence Report: CD453773

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Sequence
CD453773
Contig
Ta.28866.2.A1_s_at
NSFT03P2_Contig13614
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC282036
wEST map position
CD453773
NCBI UniGene
Ta.34636
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001068268.1 Os11g0614400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2BL
2DL
Clone Library
CS wheat salt-stressed crown cDNA library
Tissue
Crown
Developmental Stage
Adult plant
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
WHE2106_B01_C02ZT CS wheat salt-stressed crown cDNA library Triticum aestivum cDNA clone WHE2106_B01_C02, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2106_B01_C02
Probe
WHE2106_B01_C02
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttgtatctagagggtggtagcgaacacaactcag
attttatttaaaaataataattacccacccacattacattatattacaac
tctcgacctagctatagcatagctagggatgcaaatggacagcgtcgacg
tttgtccatgaaagcataattaaaacatgcacggttattaaaataacaaa
gattatcggccgtttgcatccttaagcatagcagctagctagtgcttctt
cgtctctcttctctttcgctcaaatcgtcctcttctctatttggagttga
ggttggtttggcgcagcttgcgctcctcagagagcttcctggcgaagcgc
gtgagggcctcttcgttggtgccctcaccttcgacggattcatacatccc
tgtgtcaatgttcacccgggacacattcttcttgagcagattcttaccga
tcccgataagcgcctccatgttctctttggtggcgatatcgaccgaggat
gtgtgccccgtgagcgagtcgtcctggatgcgtaggtagttcttctcaat
gcggagggcctgaaacagcacggcggcgtggatgtcgaccatgtcggcgc
tcgcatgggagaagatgtcgatgagcggggtgaagccgccgtcatagagc
catcgaaggacgccccacttggcgcagtcgggcgcggtgtacttctccgc
catcttggcggacccgtgccgatg

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