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GrainGenes Sequence Report: Ta.2797.1.S1_at

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Sequence
CD453972
Contig
Ta.2797.1.S1_at
Ta.2797.1.S1_x_at
NSFT03P2_Contig18295
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC279244
wEST map position
CD453972
NCBI UniGene
Ta.50525
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'ADP-glucose pyrophosophorylase preprotein'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL
1BL
1DL
4AL
Clone Library
CS wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
WHE0932_F10_L20ZT CS wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0932_F10_L20, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0932_F10_L20
Probe
WHE0932_F10_L20
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttacacgaacatccccacgggagtttgtttgctcg
cggccacttctttacctcaacacacagcctcttcttatttattacagggg
caaaacgaacgactattactttgttccactgctacttactacttactact
tactactgatgctgcctgcgcgcgcctccactttcagtctcagccattca
aagcttcaaacggcccccgtccccgggtcactcctccagtcccggccccg
agaagccgacgacaacgatcagcgcagcgcagaggttgtcgcggaacccc
gtcgcgccggcccggcggcgctctacacgacggtgccgtccttgatggtc
gcgttcttctggatcaccct

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