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GrainGenes Sequence Report: CD453986

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Sequence	CD453986
Contig	Ta.24405.1.S1_at NSFT03P2_Contig15345
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC281881
wEST map position	CD453986
NCBI UniGene	Ta.54553
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001064883.1 Os10g0483000 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	1BL
Clone Library	CS wheat 5-15 DAP spike cDNA library
Tissue	Spike
Developmental Stage	Adult plant
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE0946_B02_C04ZT CS wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0946_B02_C04, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0946_B02_C04
Probe	WHE0946_B02_C04
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttttttttttttttgggggcaaccaaaagaggca tgtaatttgatgcaaaattccaccaacatatcagacgaaactcgcgtaca ttaaaaaaccggtccactggcacaaataaggtaggtcaaaattcttgaaa ggggaggtggtctctaatggcgccgcttaacttataacccttcacaacag taaacatgcatcaaaatcacgactctgcctttttgtcttcagcggcctct tcaaatgtctctccagggacaagctcaggaacatcatcgtcgtcgtcagc ctgagcagctcctgcactggtaccagcctcgatgccagcagcaccaccag ggacctgcttttggaattgctctgcgagcctccgcaggttgtccaagttg tcaggaccaagttgattgataattgatggcaacagatcttgcagtttctt tgtctgtggagttccactgaccacccatgtattagcaccaatcgaagctt gcactttaggattcaaaaactgaataaccacatcatccttaaggatgttg acctcttcgataccagggatggtgttgactcctactcttttcagggtact ctggagccttttgtcatctgtagtcgtggtcttgtgtacagccttcttct tcctgggcatgctacccttccccccg

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