GrainGenes Sequence Report: CD454253
Sequence
CD454253
Contig
Ta.3348.1.S1_a_at Ta.3348.2.S1_a_at NSFT03P2_Contig10060
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC334681
wEST map position
CD454253
NCBI UniGene
Ta.3348
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_565289.1 ATUBC2 (UBIQUITING-CONJUGATING ENZYME 2); ubiquitin-protein ligase [Arabidopsis thaliana]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1DL
Clone Library
CS wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title
WHE1768_A04_A08ZT CS wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1768_A04_A08, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1768_A04_A08
Probe
WHE1768_A04_A08
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A ) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttaaacaaatccaaattgtgctcgatctg
attcaaaggcagcatggtatgttatatgtagactggatacaataatctcc
atgctgagcgtctaaaaatgtcactcccaacaacatacacgacaaagcat
gatgtaacattttaattaccttgcaagcagttcagtacataggactcaag
gcgctaacgggcaattagctggcatcacaactggatgcagcgactttagt
ctgcagtccagctttgctccacaacctcgcgtactttagggttgtattcc
cgcttgttctcgctgaataggcgggcggcttctgagttggcaggtgaatt
tgggtttgggtcccaaagcagtgactgaatggatgtcaatatagcagcta
catcatatatcgggctccactggttttggagaatatctaaacatatgctt
ccatctgcataaatattgggatgaaacattcgaaaaacaaaccgcactgt
aggtggcttattaggataatcttcagtaaactgaagtgtcagcttgaacg
taccaccatccca
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CD454253
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
|
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||