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GrainGenes Sequence Report: NSFT03P2_Contig17648

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Sequence	CD454537
Contig	Ta.2251.1.S1_at NSFT03P2_Contig17648
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC277251
NCBI UniGene	Ta.2251
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Delta-24-sterol methyltransferase'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	CS wheat pre-anthesis spike cDNA library
Tissue	Spike before anthesis
Developmental Stage	Adult plant
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE2325_G10_M19ZT CS wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2325_G10_M19, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE2325_G10_M19
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A ) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttttttgggaagaaaatgcatctagcttatttat gccactaatcgcttagcacaatggcgtcaaacagtcagttaatcgacgag gatcaaaagctcgcacgacaaaacaacaaaacagaagctcaacattaatt aaatacaaaacaaacagaaacgcaacacatttcgctacatctcttctaca gataagaaccgaggatcgccatagccaaactcctcggtcgagcccgcttc ttcaaaatgcatctttgcattgcctgaaagttgctcattctgaaagaggc ttccgaaccacaaagaagtacatcggagtgaagatctccttcttgccacc ttcgacaagaccttccgcggccttctctaagaaactagagaccctctggc tgccttccggagccagaccaacatattccaataccttgaccatattgcga gtaattattcgtcccacggtagtcaaccggaaactactcaatgaaaaccg acttgggtccaagggcaagtaccaaggcaacggagaatcttcagcaagat ccttgtcccaaataacctcaaacccagcatctttaacagcttgaagacat tgccgagtacttctgatatctggcagaccattcccaagctcaatttcatc cttaatcctcttgtgggttgcattgtttggatcatagtgatcggtaa

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