GrainGenes Sequence Report: CD454901
Sequence
CD454901
Contig
Ta.23960.1.S1_at NSFT03P2_Contig18815
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC277889
wEST map position
CD454901
NCBI UniGene
Ta.55119
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to XP_001690893.1 small ARF-related GTPase [Chlamydomonas reinhardtii]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4DL 5DL 6BL
Clone Library
CS wheat heat stressed spike cDNA library
Tissue
Whole spike
Developmental Stage
Spikes at 5, 10, 15 and 20 days after anthesis
Data Source
genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title
WHE1713-1716_E12_E12ZT CS wheat heat stressed spike cDNA library Triticum aestivum cDNA clone WHE1713-1716_E12_E12, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1713-1716_E12_E12
Probe
WHE1713-1716_E12_E12
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Spikes at 5, 10, 15 and 20 days after anthesis were heat stressed under two conditions at Texas Tech University (D. Zhang in HT Nguyrn lab): (1) at 38 C for 4 hours and (2) 5 days of cyclic treatment of 38 C for 4 hours. Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttagataaatgcttgaacacttattacgaaatt
gaacaggactctctttagctgtttacttcagaaacatcttagggctcaaa
atacaataaaacaatagtatgacagtgaaaccacagccggagctggctca
gtaactgttgatctcaggaaaatacacagtacatacgggaatagcatagc
accatcccatcccaacacaatccctaggtacccggtgattcaatcgtcca
tccaaagcgctcctagctccttcaaaatcttgacgaaaatttaggacttg
ctggcaatgttgctggacagccagtccaggccttcatacagtccctcccc
tgttgtagcacaagtgctctggatgtaccagtgtcgctggcgaagggagt
gcaggccaagcttatcagtgatctcagcagcattcatggcatttggaaga
tcttgcttgttagcaaacacaagcagcacagcatcacgtaactcatcctc
attcagcatcctgtggagctcatcccttgcttcaacaacacggtccctat
cgttgctgtccacaacaaagatgagaccctgtgtgttctggaagtaatgc
ctccacagtggcctgatcttgtcctgacccccgacatcccagacagtgaa
gctgatgttcttgtactccacagtttcaac
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CD454901
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||