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GrainGenes Sequence Report: CD454943

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Sequence
CD454943
Contig
Ta.26047.1.A1_at
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC320097
wEST map position
CD454943
NCBI UniGene
Ta.26047
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, weakly similar to NP_001062379.1 Os08g0539700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
6BS
Clone Library
CS wheat drought stressed leaf cDNA library
Tissue
Leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
WHE2015_C02_F03ZT CS wheat drought stressed leaf cDNA library Triticum aestivum cDNA clone WHE2015_C02_F03, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2015_C02_F03
Probe
WHE2015_C02_F03
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 80%, 70% and to 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttaggaacaaataattgataagtagttctgcaa
tgactactgactcaaaaattgaagtcaagccacagatttgtaaacctgga
agtcaacgacataggaagagttgtgaaatttaattcaatgtgaagaacaa
acatcttcatatgtcagcatttcggtatctactatagtacgtagaataca
gtagcaagtgcgcacacgaatccagagagagagacaagagagtttagtca
acaaaattcactctcacgtgcttcatatgctccacctgcctctccacgtc
tttcttgagttcgctattagacaaccgaatttccttgaggcttgtgagga
ccgctggcagcccggagaatccattcagctcaacacacaaatcaacttgt
agcagctcgagcttaggcaatgcgtcttcttcaaacgacaccgactcgag
gatgtataatcgtgagagatgtagctccatgagactcgggaaagatgagc
tccggaattggagatgtgtccccgtgaacgcatcccagaggcgtaggact
gcaagatttggtaggaacccaagggcttgtatggaatcttgcctcaagta
gctctcccgtagcaccagcttggagagattctgaagcatatggatccatt
ttggtacattggttagcctaccttc

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