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GrainGenes Sequence Report: NSFT03P2_Contig1373

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Sequence	CD490492
Contig	Ta.25511.1.A1_at NSFT03P2_Contig1373
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC365844
wEST map position	CD490492
NCBI UniGene	Ta.25511
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001048888.1 Os03g0135600 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Brevor
Chromosome	4BL 5AL
Clone Library	Brevor wheat dormant embryo cDNA library
Tissue	Seed embryo
Developmental Stage	Mature seed
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE2954_B02_D04ZT Brevor wheat dormant embryo cDNA library Triticum aestivum cDNA clone WHE2954_B02_D04, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE2954_B02_D04
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown to seed maturity under conditions favoring seed dormancy (L. Dohery at K. Walker_Simmons lab, Washington State University, Pullman, WA). Embryos were cut from mature dormant seed (Doherty). Total RNA was prepared from these embryos, polyA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Chin, Fenton). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tttttttttttttttttttttttttttttttgctcttctgaactctatta ctgcttgcattctttggtctctggaagtataggaacggtataacaaccat agctggcttgaccttaagcaggtgtagtggctaattccgaaaataccagg aattcgaaaagcctctacatggagagcatgatcccttcgatagacagatt ctgtcatcgcatcttccatttgctggggatgccctcacagctggaatgag gtgtacatgatgatgctggccttcatcagatttctctggaccttctaggg aaatgctccaaactgctaatcactgaagaccgtgagtggcctgctaactc ctcgcatgctcagctcatggagatcttccctggaagccaacccagcgtcc attctggagccttgctctgtcgccacaggctctcacacccactgaagaag ctgcgcatggaacctgaatcgatcaacaacctgtgctggagccgcctgaa actgtctgagagttctctcctgtattgtaatctactacctggcaacagtg actggtctatctatagtttgtgacggcccagtggctacctctaatctttc tgcatcatcaaccttttccttgtgaaaagatttgctttccagttttcgtg gatagaaaataaccaggtagttcatttctctccagcagcaaacatgtcct atctgcaactggaagcaattccgtcatg

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