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GrainGenes Sequence Report: CD491122

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Sequence	CD491122
Contig	Ta.25362.1.A1_at NSFT03P2_Contig8941
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC319837
NCBI UniGene	Ta.25362
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001059634.1 Os07g0479300 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	CS wheat cold-stressed seedling subtracted cDNA library
Tissue	Seedling
Developmental Stage	Five-day old seedling
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE3074_B07_D14ZT CS wheat cold-stressed seedling subtracted cDNA library Triticum aestivum cDNA clone WHE3074_B07_D14, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE3074_B07_D14
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before subtraction was carried out. The mass excision of phagemid library and subtraction were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before subtraction was carried out. The mass excision of phagemid library and subtraction were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttttttttttttttttagcacaagccataagtat tataatagcatacaagacctggacataaacagccctcataacaaagagat tggcttctgtccataacaattctaagctttattacatgctaacaatcgag tggcgtcaccgatacttcagtgactcaagcaccgtacaagggaaataaca tctgtacggtagcagtgagcttgtcatcacctaaaatcattctgacgtgc ctactaagcactttatactaatttatggggaaaccatgttcctctgatcg tgtcacaagaggacagtgtgtatatcacatggtaaaatcgagcctctgga agctcgaggatgcgttggaaaggtttcctgaggtccacctcttcagcatc tccagagcgaccttcggttgatccattggtaccatgtggccagcatcgtg gaccttcaagaaactcaaaggtccatagcttttcagaataccagcttctt ttccatcaactgtgaaaggttcctcagatgaggacacaaaagcctccttt ccagaccattccatagaattcacccatcttcct

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