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GrainGenes Sequence Report: CF132947

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Sequence	CF132947
Contig	Ta.9552.2.S1_at
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC356120
NCBI UniGene	Ta.43347
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_565111.1 nodulin MtN21 family protein [Arabidopsis thaliana]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat meiotic floret cDNA library
Tissue	Whole florets with anthers
Developmental Stage	Meiotic stages pre-meiosis-metaphase I
Data Source	genbank Release 137, Aug 15 2003 genbank Updated Nov 2006
Title	WHE4352_C01_E02ZT Wheat meiotic floret cDNA library Triticum aestivum cDNA clone WHE4352_C01_E02, mRNA sequence.
Strain	lab_host E. coli DH10B
Clone	WHE4352_C01_E02
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T7 primer. Note: Vector: pSPORT1; Site_1: SalI; Site_2: NotI; Plants were grown in a glasshouse. Anther meiotic stage was determined microscopically after removing a single anther from a primary floret. If determined to be between (and including) meiotic stages pre-meiosis and metaphase I, remaining floret tissue was collected and pooled for library construction. The tissue, total RNA, and poly(A) RNA were prepared, cDNA synthesised, and directionally ligated into pSPORT1 by Tim Sutton in the P Langridge Lab at the Department of Plant Science, University of Adelaide, Waite Campus, Australia. Average insert size 1.4Kb. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tggtacgcctgcaggtaccggtccggaattcccgggtcgacccacgcgtc cgatagctagacacccctggttgcactcctcactcacctcatccctggcc taggccttcctctctctagtctctcatcgccacctcgctccagcagcagc ttctatctcgatctagggagagatcaagtgaggagagggtgcagccatgg cggacgcggtggatgctcgccgggtctgcgggatgccggagaaggcgcag ctccacgtggcgatgctggcgctgcagttcggctacgccggcttccacgt cgtgtcccgcctcgcgctcaacatggggatcagcaagctcgtcttccccg tctaccgcaacatcatcgcgctcttcctcctcgtccccttcgcctacttc ctcgagaagaaggaccgacccaggctcaccctcggcctcgccgtccactt cttcttcctcgccctctgcggcatcaccgccaaccagggcttctacctcc tcggcctcgacaacacctcccccaccttcgcctccgccatccagaactcc gttccggccatcaccttcggcatggccgccgcgctccgcatcgagaaggt gcgcctcgaccgccgcgacggcgtcgccaaggtgctgggcacgctcgcgt gcgtcgcaggcgcgtccgtcatcacgctctaccaaggccccaccatct

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