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GrainGenes Sequence Report: Ta.12521.1.A1_at

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Sequence
CK193730
Contig
Ta.12521.1.A1_at
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC329176
NCBI UniGene
Ta.9895
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus'
Keyword
EST
Species
Triticum aestivum
Clone Library
Triticum aestivum FGAS: Library 3 Gate 6
Data Source
genbankRelease 139, Dec 15 2003
genbankUpdated Nov 2006
Title
FGAS002147 Triticum aestivum FGAS: Library 3 Gate 6 Triticum aestivum cDNA, mRNA sequence.
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Wm L Crosby; Bioinformatics; University of Saskatchewan, Department of Computer Science; 1C101 Engineering Building, 57 Campus Drive, Saskatoon,; Saskatchewan, S7N 5A9, Canada; Tel: 306 966 1769; Fax: 306 966 2033; Email: fgas_ests@cs.usask.ca; This sequence is the direct result of the Base calling software; Phred (default parameters). It is the raw base calls. To aid in the; identification of the high quality insert the software Lucy; (default parameters) has been run on this sequence. Lucy identified; the region [103,633].; Plate: L3C102-3P row: K column: 05.
Note: Organ: Root; Vector: pCMV.SPORT6; Root tissue from control, cold-acclimated and salt stressed wheat cultivar Norstar. 7 mRNA populations were combined before constructing the library; 7 day non-acclimated roots, 1, 23, and 53 days cold-acclimated at 4C, and 30 minutes, 3 hours and 6 hours treated roots with 200mM NaCl. Non-acclimated and cold-acclimated plants were grown in vermiculite while salt stressed plant were grown hydroponically. First strand synthesis in this library was done in the presence of methylated dCTP thereby protecting from internal cleavage with NotI.
DNA
tttacccgagcgttcgttacgtgncttacatacgacttagtatagggacc
actttgtacaagaaagctgggtacgcgtaagcttgggcccctcgagggat
actctagagcggccgccctttttttttttttttttttttttttttttttt
ttcttccccggcacggtatattcacaggaatatgcagggtgacaggcacc
attcttacagggagcgcctgggtaactggggaggacgtagccagctggtt
aagaagagatcagtggcaactattgtggagcatattgccctagtgaagct
aagacaagatgttaatcatacttgtatagcagctagcggaccctaacata
cccaaattataggagtacctgttatcacctactccctccattccgaaatg
taagtcgctggaggattagttccgaccaggtgaaaacggtggaatttaca
tcgttgcccctagtttgaatcggtatccccatcgtcttcctccatccgcg
aggccatagcccgcccgttcaccaggccatcgtccgttcgcccgcccctg
tttctcctccgcctgcccctgcttctcctccatgcgcccaggtcccaggc
gctcccagggtcctcgcctccagcagcgatccctggtccttgtttccagc
agcgatcccagatccaggggttgccaggaggcacacagcagcagcaatcc
cagtttccnagttcagcannagtttctatagtgttcttcgtttngnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntntnncggggnnn
ttgtctttttctggttgttttttcttttccggtgcagccagttccctgnt
tggt

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