GrainGenes Sequence Report: CV056304
Sequence
CV056304
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC175802
NCBI UniGene
Hv.19370
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'MIKC-type MADS-box transcription factor AGL1'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
Himalaya
Clone Library
Barley EST endosperm library
Tissue
endosperm
Developmental Stage
developing endosperm tissue 10, 12, 15 dpa (days post anthesis)
Data Source
genbank Release 144, Oct 15 2004 genbank Updated Nov 2006
Title
BNEL16C1 Barley EST endosperm library Hordeum vulgare subsp. vulgare cDNA clone BNEL16C1 5' similar to MADS-box protein PTM5-like, mRNA sequence.
Strain
lab_host DH10B (Life Technology) vulgare
Clone
BNEL16C1
Probe
UMB606
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Feature: source: mol_type = 'mRNA' Locus Comment: Contact: Bill Taylor; Commonwealth Scientific and Industrial Research Organisation; Division of Plant Industry.; CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia; Tel: 61 2 6246 5223; Fax: 61 2 6246 5000; Email: Bill.Taylor@csiro.au; Seq primer: M13 reverse primer; High quality sequence stop: 351. Note: Vector: _ZipLox; Site_1: Sal I; Site_2: Not I; mRNA was prepared from endosperm tissues of the barley cultivar Himalaya. cDNA was synthesised from pooled 10, 12, and 15 dpa endosperm using Not I-oligo(dT)18 primer/adapter (Pharmacia Biotech), and then ligated to the Sal I-Not I site of _ZipLox vector (Life Technology) after adding a Sal I-Xho I adapter (Stratagene). Constructed by Shahjahan Ali and Bill Taylor.
DNA
cacatctcccctccctccctccctctaccagattaggcctgggccgctgc
tgctgctcctcctcctcatcactccctccctctctccctagatccgtccg
atccatggcgccgtcgtcgccggaggcggcggtgcctgaaggaggcgagc
agcagcagatcgtggcggcggcggcggcggcgggggccaaaaagggcggc
gggaagaggggccggcgcgagatgcggcggatcgaggacgccaccagccg
gcaggtcaccttctccaagcgcaggagcgggctgctcaagaaggccttcg
agctgggcgtgctctgcgacgccgaggtcgccctcatcgtcttctccccc
c
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CV056304
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||