GrainGenes Sequence Report: CV063649
Sequence
CV063649
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC190864
NCBI UniGene
Hv.18441
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'Transcribed locus, weakly similar to NP_001051252.1 Os03g0746700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
Himalaya
Clone Library
Barley EST endosperm library
Tissue
endosperm
Developmental Stage
developing endosperm tissue 10, 12, 15 dpa (days post anthesis)
Data Source
genbank Release 144, Oct 15 2004 genbank Updated Nov 2006
Title
BNEL91f7 Barley EST endosperm library Hordeum vulgare subsp. vulgare cDNA clone BNEL91f7 5' similar to unknown protein, mRNA sequence.
Strain
lab_host DH10B (Life Technology) vulgare
Clone
BNEL91f7
Probe
UMB709
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Feature: source: mol_type = 'mRNA' Locus Comment: Contact: Bill Taylor; Commonwealth Scientific and Industrial Research Organisation; Division of Plant Industry.; CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia; Tel: 61 2 6246 5223; Fax: 61 2 6246 5000; Email: Bill.Taylor@csiro.au; Seq primer: M13 reverse primer; High quality sequence stop: 717. Note: Vector: _ZipLox; Site_1: Sal I; Site_2: Not I; mRNA was prepared from endosperm tissues of the barley cultivar Himalaya. cDNA was synthesised from pooled 10, 12, and 15 dpa endosperm using Not I-oligo(dT)18 primer/adapter (Pharmacia Biotech), and then ligated to the Sal I-Not I site of _ZipLox vector (Life Technology) after adding a Sal I-Xho I adapter (Stratagene). Constructed by Shahjahan Ali and Bill Taylor.
DNA
tttttttttttttttttttttttttttttttttttttacagacaaaaccc
aatctgtttgatcatctgatccaagaagcatgatcatttcatcaagatcg
atccaagaacaaattttaggacgacaagactatggcagcatcaacaagac
ttactactttaccggagcacgcacgcacgcacgcattatggaggagagga
cttgctactatggatggcaggcatggcgtcacaggctgaggaaacctttg
ccctggcgcgccggcgccggcggggggcttcccacgacgcggtcgccctc
cacgacgcggtcgccgaaaatgtgcaccgtccgcgggttgggcccgcggg
agatgacgcaggtgtagtcctcggacgacggcccgttcttcttcgcctcc
gccgccgccgggcgggcggggcgcaacggcagccacgagctcttgttttt
gacgccgaactccacgcgccggtccagggaggaggagcgcaccagggccg
gcgcctgcgccctgacggcgccccggaggacgctcttcctccgctgggcc
tcgtgggcgcagtccagcacgttggggagcccgtgcggccccgcgcaccc
gtngaaccacgggcggngcttgcccccgccgggctccacggncgggccgg
gcgacccgaaagccgccgacgcgttcaagcacgatgtcgggctcatggcg
taagcggacatcgcctt
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CV063649
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||