GrainGenes Sequence Report: CV063745
Sequence
CV063745
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC187369
NCBI UniGene
Hv.593
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'CDNA clone: FLbaf179b01, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
Himalaya
Clone Library
Barley EST endosperm library
Tissue
endosperm
Developmental Stage
developing endosperm tissue 10, 12, 15 dpa (days post anthesis)
Data Source
genbank Release 144, Oct 15 2004 genbank Updated Nov 2006
Title
BNEL92g10 Barley EST endosperm library Hordeum vulgare subsp. vulgare cDNA clone BNEL92g10 5' similar to putative ribosomal protein S29, mRNA sequence.
Strain
lab_host DH10B (Life Technology) vulgare
Clone
BNEL92g10
Probe
UMB710
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Feature: source: mol_type = 'mRNA' Locus Comment: Contact: Bill Taylor; Commonwealth Scientific and Industrial Research Organisation; Division of Plant Industry.; CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia; Tel: 61 2 6246 5223; Fax: 61 2 6246 5000; Email: Bill.Taylor@csiro.au; Seq primer: M13 reverse primer; High quality sequence stop: 520. Note: Vector: _ZipLox; Site_1: Sal I; Site_2: Not I; mRNA was prepared from endosperm tissues of the barley cultivar Himalaya. cDNA was synthesised from pooled 10, 12, and 15 dpa endosperm using Not I-oligo(dT)18 primer/adapter (Pharmacia Biotech), and then ligated to the Sal I-Not I site of _ZipLox vector (Life Technology) after adding a Sal I-Xho I adapter (Stratagene). Constructed by Shahjahan Ali and Bill Taylor.
DNA
caaaggtagggtttacagttcccaacttccaagcctcgccgccgccgccg
ccgccgccatgggacactcgaacgtctggaactcgcaccccaagaactac
ggccccggatccagggtttgccgtgtctgcggcaactcgcatggcctgat
ccgcaagtacgggctgatgtgctgcaggcagtgcttccgcagcaacgcca
aggacatcggcttcatcaagtaccgttaagaatggaaacctcaaaggaag
caaggactaggaaattatcatcgaactgcggtgcttcatcgaggccgatc
tttattatcctataaactatgtaatgaactactgttaatgtttgaattgg
agtgacaaatccgtgaaaccatgttggtgactgctgtttgagcatctgaa
acccgtaattctgttactgtcatgcctttatgtttnaaaaaaaaaaaaaa
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
aaaaaaaaaaaaattttcct
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CV063745
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||