GrainGenes Sequence Report: CV064891
Sequence
CV064891
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Species
Triticum aestivum
Cultivar
Hartog
Clone Library
Wheat EST endosperm library
Tissue
endosperm
Developmental Stage
developing endosperm tissue 6, 8, 10 dpa (days post anthesis)
Data Source
genbank Release 144, Oct 15 2004 genbank Updated Nov 2006
Title
WNEL16e8 Wheat EST endosperm library Triticum aestivum cDNA clone WNEL16e8 5' similar to plasma membrane H+ ATPase, mRNA sequence.
Strain
lab_host DH10B (Life Technology)
Clone
WNEL16e8
Probe
MAG1691
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Feature: source: mol_type = 'mRNA' Locus Comment: Contact: Bill Taylor; Commonwealth Scientific and Industrial Research Organisation; Division of Plant Industry.; CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia; Tel: 61 2 6246 5223; Fax: 61 2 6246 5000; Email: Bill.Taylor@csiro.au; Seq primer: M13 reverse primer; High quality sequence stop: 810. Note: Vector: _ZipLox; Site_1: Sal I; Site_2: Not I; mRNA was prepared from endosperm tissues of the wheat cultivar Hartog. cDNA was synthesised from pooled 6, 8, and 10 dpa endosperm using Not I-oligo(dT)18 primer/adapter (Pharmacia Biotech), and then ligated to the Sal I-Not I site of _ZipLox vector (Life Technology) after adding a Sal I-Xho I adapter (Stratagene). Constructed by Shahjahan Ali and Bill Taylor.
DNA
tacaatatcatcacatacctcccgctcgatatcatcaagtgcctcatccg
atacactctgagcggcagagcgtgggacgaagacggggaccaagaaaagc
aagacgagcgtggagacgtacaagatgtacatctctaaggagctgaagtg
ggcacacgctgacaggaccctccatgggatggacacgtccattgtgaact
tgttcatcaacgacatcttctagaagctcgccggcgagatggctgaagag
gccaagaggagggccaagattgcgatgttgagggaggttcttccgctcta
cgggcgcgttggactctacggacagcgtaagggtctcgacatcgagacga
tccagcagtcctacacggtctgatgctctgagctgaccatgagcgatgag
tgggtccttgtatgtgctgacttctcgtgaatcggtcgatgaatcgtgcc
gcgtggtatctgaagacaataactttactggtagtttanaaacggttggn
tcccggttggctggaacaattacgtatttcggctgcgcaacgcattatag
tgggaagtgggccaatacggtcgcggtggcttcgtctctgcgttttagga
acccttgggttaatttaatagacccctctgacacctatgggtttgcagca
atgggctataccctgctgcttctagttacgctgggctccggtggtatcaa
cgtttcggttggaggtcgtctctctctgagtcggactcccggctatccat
tttgcggtctctttcctcgcacctctgtaccctttttcgtgggcgcggac
cccgtcttcg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CV064891
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||