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GrainGenes Sequence Report: BE352611

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Sequence	BE352611
Contig	NSFT03P2_Contig2206
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
wEST map position	BE352611
DB Remark	Locus Source: Triticum aestivum (bread wheat)
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	5AL 5BL 5DL
Clone Library	Wheat etiolated seedling root cDNA library
Tissue	Root
Developmental Stage	Five day old etiolated seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0425_F12_K23ZS Wheat etiolated seedling root cDNA library Triticum aestivum cDNA clone WHE0425_F12_K23, mRNA sequence.
Other Name	WHE0425_F12_K23ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA008E1X
Clone	WHE0425_F12_K23
Probe	WHE0425_F12_K23
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Strategene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	accattagtgaaatgatggaagagttgtttgtggcacaatttattggtca gggtgaggtgtcagggtcaggtgtaagggcatagttcgagaagagctgct ggagatagtttttttttcctttgaaaccattttcctcacatcaagttgcg tataatcattttcatggtaatagttcaagtcatgtatacataagcatgag cttttcctttttgtaatgataattaggtacaagctgaagagaagatttga tcgcggttcatatggtgaagtctggttggcatttcgttggaactgctctn gatgatgtagatgtacataaagatccttcccactttagcactatacttac accagactcatataattgcacaagttcaaacacaacatcgtcatctgatg aaaatcatggctcagacatgatagatgggtgatttattcatattgaagcg cataatggtggaaagaggaaatgctgcttacttgagtggattgcgggaga agtattttgggagaattattttcaaatgcttcaaaaactctttgaagttt tgtcaaggatggaatcatcatctgcaacgtttccgatggacatgcaattc attgagtatacctttcca

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