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GrainGenes Sequence Report: NSFT03P2_Contig15444

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Sequence	BE403428
Contig	TaAffx.42245.1.S1_at NSFT03P2_Contig15444
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC280238
wEST map position	BE403428
NCBI UniGene	Ta.35381
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001050453.1 Os03g0439500 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	1AL 1DL 3AL 3BL 3DL
Clone Library	Wheat etiolated seedling root cDNA library
Tissue	Root
Developmental Stage	Five day old etiolated seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0429_D11_G21ZS Wheat etiolated seedling root cDNA library Triticum aestivum cDNA clone WHE0429_D11_G21, mRNA sequence.
Other Name	WHE0429_D11_G21ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA008E1X
Clone	WHE0429_D11_G21
Probe	WHE0429_D11_G21
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Strategene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	TaAffx.42245.1.S1_at 969 WHE2AFFY TaAffx.38803.1.S1_at 246 WHE2AFFY TaAffx.38803.1.S1_at 147 WHE2AFFY
DNA	gcgcccacggaggggagagtcgcaggatccatggagcgtatcggcgacaa ggtgatcagcagggccgagatcacggacgccgcgtcggccacgttcgcgg actccactgtgatccccgacaggtatgcccggccagatgaggtcagggac ggggtcgtcgtcggcagcgacgagagctatgagctaccggttgtagacat ggcgaggctgcttcacccggagttgtcggaagccgagattgccaagcttg gctctgcgtgtcgagactggggcttcttccagttaacaaaccacggagtt gatgaaacagttgtacaagatatgaaagataacactctgcagttctttgg cttgccgctggagaagaagaaggcagtggccatccaagccaatggcttcg aagggtttggacaccactacaacagagcgtcgtctgaaaagttggactgg gcagagagcctaatcctcgtgacgcagcaacacgagcaaaggaacattca gttttggcctgccaatccgtcgacatttagggatgcacttgacaagtact c

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