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GrainGenes Sequence Report: BE405167

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Sequence
BE405167
Contig
Ta.3308.1.S1_at
NSFT03P2_Contig15016
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC301435
wEST map position
BE405167
NCBI UniGene
Ta.3308
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001048802.1 Os03g0123100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL
1BL
1DL
Clone Library
Wheat etiolated seedling root cDNA library
Tissue
Root
Developmental Stage
Five day old etiolated seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1211_F08_L15ZS Wheat etiolated seedling root cDNA library Triticum aestivum cDNA clone WHE1211_F08_L15, mRNA sequence.
Other Name
WHE1211_F08_L15ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA008E1X
Clone
WHE1211_F08_L15
Probe
WHE1211_F08_L15
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Strategene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.3308.1.S1_at918WHE2AFFY
[ Show all 7 ]
DNA
acgcatcgtcgtctgcgaatactgcgaaacccaaaccccagactcagcga
cagagagcagccagtagcctcctccgccgccgcccatgtcaggaggaggg
atcgcgcggggccgcctcgcggaggagcgcaaggcctggcgcaagaacca
cccccatggattcgtcgcgaagccggagacggtggccgacgggtcggtga
atctcatgatctggaactgcaccatccccggaaagcaggggactgattgg
gaaagtggatactacccacttactctccacttcagtgaggactaccctag
caaacctcccaagtgcaagttcccgcagggttttttccacccaaatgtct
atccttcagggacggtctgcctctcgattcttaatgaggatagtggttgg
agacctgccatcactgttaagcagattctagttggaatacaggatttgct
tgatcaacctaatccagctgatcctgcccagactgatggttatcacctc

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