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GrainGenes Sequence Report: BE423707

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Sequence
BE423707
Contig
NSFT03P2_Contig3864
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
NCBI UniGene
Ta.56565
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus'
Keyword
EST
Germplasm
CHEYENNE
Species
Triticum aestivum
Cultivar
Cheyenne
Clone Library
Wheat endosperm cDNA library
Tissue
Endosperm
Developmental Stage
5 to 30 days post anthesis seed
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0073_E12_J23ZS Wheat endosperm cDNA library Triticum aestivum cDNA clone WHE0073_E12_J23, mRNA sequence.
Other Name
WHE0073_E12_J23ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA001E1X
Clone
WHE0073_E12_J23
Probe
[Wcl275ct941cn1388]
{SpCl-1243}
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Seeds collected, endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab.
DNA
gagcgtcatgcatttattgaatatgcaggccaaaattccatttgcgttca
cagatctcaacgcacgccgccggcgcgccgctcacctcaccaagaacgca
aacaacagctctatcttctctgctaattagattctacagacagaacacgc
ggggaaaatcctaggagaaaatccgacgaccaaaattgggcccgcgtccg
gcagagggtacacgggtgcgcgcgccgcgccgcgccgcgcctacgacgcg
tcctccccgcgcggctcccccgcgtcggcggcggtcacggcgggggaagg
cgaacggcctgncgctggcgaggaaggacggcaagtcgtcgccggccatg
atgacgacgaccttgggctccctgtcgagcggcgccatcccgcgcgccga
cgcgtcctcgccggcgctcccccgccgcgacgacgacccggacggcttgc
tgtccgtgcagacgagcacgagcagcgccacggcgatgatccccatcatg
agcgcgaagc

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