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GrainGenes Sequence Report: BE423752

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Sequence
BE423752
Contig
TaAffx.10350.1.S1_x_at
NSFT03P2_Contig18590
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC312771
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Germplasm
CHEYENNE
Species
Triticum aestivum
Cultivar
Cheyenne
Clone Library
Wheat endosperm cDNA library
Tissue
Endosperm
Developmental Stage
5 to 30 days post anthesis seed
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0073_A08_B15ZS Wheat endosperm cDNA library Triticum aestivum cDNA clone WHE0073_A08_B15, mRNA sequence.
Other Name
WHE0073_A08_B15ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA001E1X
Clone
WHE0073_A08_B15
Probe
[Wcl53ct224cn331]
{SpCl-29}
BE423752
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Seeds collected, endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab.
DNA
tttttttttttagcacagtacaggggcccagtaccaagaaacacagacga
gttacatcaaagttcagacttcggaacatggtccctctcctcactctctc
caacactctcttcttcctcgcagaaacgccggctcaatcattcaatcaca
aggcgacacacgcacacagacaccattgccacacatcgcacacggtttta
tttggacacagcaacattattcgctaccagcggcagaggactacgacgac
gacggacggacggacggacggacggcgactaggacgacttggggggcttg
tccccggactaatccatggcgatgtgtcgatggcaatgcggcagctcatc
taccaggggaggatcttccagcgctggttgtcgccctcgcaccacttcca
gagcacgatctcggtgccgtcgtggacgccgccgtgggccttgtcgccgt
ggaa

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