GrainGenes Sequence Report: BE445424
Sequence
BE445424
Contig
Ta.23348.2.S1_at Ta.23348.3.A1_a_at NSFT03P2_Contig15571
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC288269
wEST map position
BE445424
NCBI UniGene
Ta.55671
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001132453.1 hypothetical protein LOC100193909 [Zea mays]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5BL 5DL
Clone Library
Wheat etiolated seedling root normalized cDNA library
Tissue
Root
Developmental Stage
Five day old etiolated seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1136_B06_D12ZS Wheat etiolated seedling root normalized cDNA library Triticum aestivum cDNA clone WHE1136_B06_D12, mRNA sequence.
Other Name
WHE1136_B06_D12ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli DH10B
DNA Library
TA008E3N
Clone
WHE1136_B06_D12
Probe
WHE1136_B06_D12
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.23348.2.S1_at 733 WHE2AFFY Ta.23348.2.S1_at 684 WHE2AFFY TaAffx.119523.1.S1_at 167 WHE2AFFY Ta.9211.1.S1_at 64 WHE2AFFY
DNA
gaccttattctcgtacagcctccctatatgattgcattggcttgtatata
cattgccagtgttcttaaagataaggatacaacttcatggtttgaagaac
ttcatgttgacatgaacattgtgaagaatatttcaatggagatactggac
ttctacgaaacctacaaggttgacccccaaaggggctctccgacgaaaag
atcagcccgataatgaacaagctgccagcaaaggcctaaagtagatgttg
ttctagttaatagtaaattcttagacctactattgcaagtgaccagcgga
tcaattgtgatgcatcttttcagaatgaagaaacccatgatagggaatag
cttggatgcgcttgtagtagtctgtgatacttgacatttataactgctgt
atttacaaaacaaaattacttcaaaaaaaaaaaaaaaaaaa
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE445424
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