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GrainGenes Sequence Report: NSFT03P2_Contig18817

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Sequence	BE470825
Contig	NSFT03P2_Contig18817
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC315647
NCBI UniGene	Ta.33558
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Alpha tubulin-3A'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat drought-stressed seedling cDNA library
Tissue	Seedling without endosperm
Developmental Stage	Five day old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0281_C05_F10ZS Wheat drought-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0281_C05_F10, mRNA sequence.
Other Name	WHE0281_C05_F10ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA005E1X
Clone	WHE0281_C05_F10
Probe	BE470825
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated for one day at 90% RH. After removing endosperm, seedlings were transferred to desiccator jar containing saturated MgSO4 at room temperature for 24 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated for one day at 90% RH. After removing endosperm, seedlings were transferred to desiccator jar containing saturated MgSO4 at room temperature for 24 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ggcgtcttcgtactcgcctctctctcgcgcttccgagctttgtccttcgt cctcctcccccgacctctccagcagcgtagcgtagcgcccgcttccgccg ttccgacccgccgccatgagggagtgcatctcgatccacatcggccaggc tggtatccaggtcgggaacgcgtgctgggagctctactgcctcgagcatg gcattcagcctgatggacagatgcccggtgacaagactgttgggggaggt gatgatgctttcaacaccttcttcagtgagactggtgctgggaagcatgt cccccgtgctgtctttgttgatcttgagcccactgtgattgatgaggtga ggactggcacttatcgccagctcttccaccctgagcagcttatcagtggc aaggaggatgcagccaacaactttgcccgtggtcactacaccattggcaa ggagattgttgacctatgcctggaccgtatcaggaagcttgcagacaact gcactggtcttcagggattcctcgtcttcaatgctgttggaggtggaact ggctctggccttggttctcttctcctggagcggctctctgttgactatgg aaa

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