GrainGenes Sequence Report: NSFT03P2_Contig16961
Sequence
BE488358
Contig
Ta.9350.1.S1_at NSFT03P2_Contig16961
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC327707
wEST map position
BE488358
NCBI UniGene
Ta.10155
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wdk3c.pk006.l8:fis, full insert mRNA sequence'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2AL 2BL 2DL
Clone Library
Wheat unstressed seedling shoot normalized cDNA library
Tissue
Etiolated shoot
Developmental Stage
Five day old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1055_H09_O17ZS Wheat unstressed seedling shoot normalized cDNA library Triticum aestivum cDNA clone WHE1055_H09_O17, mRNA sequence.
Other Name
WHE1055_H09_O17ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli DH10B
DNA Library
TA006E2N
Clone
WHE1055_H09_O17
Probe
WHE1055_H09_O17
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.9350.1.S1_at 733 WHE2AFFY Ta.9350.1.S1_at 218 WHE2AFFY Ta.26119.1.A1_at 115 WHE2AFFY Ta.26119.1.A1_at 56 WHE2AFFY
DNA
gggaaactatgctcggtggacaaggcgaatgtgcttgaggcatctatgct
ttggaggaaacgggtaactgcaatagcttccgaattccccgacgttgagc
tctcacacatgtacgttgataacgctgcaatgcagcttgttaggaatcct
aaacagtttgacacgattgtgacaaacaatatctttggtgacatattatc
ggacgaagcatcaatgatcacaggaagcattggaatgctgccatctgcca
gtgttggtgaatcgggaccgggtctttttgaacccattcatggctctgct
cctgacattgctggccaggacaaggcaaacccgttagctacaattcttag
tgcggcgatgctattgaaatatggccttggtgtagaaagtgctgcaaaga
gaatcgaagccg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig16961
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