Query (optional)   in Class  

GrainGenes Sequence Report: BE488792

Sequence
BE488792
Contig
Ta.7929.1.S1_at
NSFT03P2_Contig15378
Tracefile
[ Download ]
[ View ]
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC278827
wEST map position
BE488792
NCBI UniGene
Ta.54767
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001063826.1 Os09g0543100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5AL
5BL
5DL
Clone Library
Wheat unstressed seedling shoot normalized cDNA library
Tissue
Etiolated shoot
Developmental Stage
Five day old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1054_G10_N20ZS Wheat unstressed seedling shoot normalized cDNA library Triticum aestivum cDNA clone WHE1054_G10_N20, mRNA sequence.
Other Name
WHE1054_G10_N20ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli DH10B
DNA Library
TA006E2N
Clone
WHE1054_G10_N20
Probe
WHE1054_G10_N20
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.7929.1.S1_at839WHE2AFFY
Ta.7929.1.S1_at757WHE2AFFY
DNA
atcctcacggccaggtatatgggtataggagctccctctgggctgaacac
cttggtatggttgatgaccacttcaaggaaccttctagcctggactgtgt
aagattagtgaaccaaatagccgaagaaaactggaaagattcgcgtcaga
agagatgcaaacgttgcaagggcacctcctcagatacccggtgaagggag
agcccgatggtaagatcgtcccgctgcccgaccaagaatgcttccccgac
gttggcggcaagatctgcggcgctccgacctcgcttcctgattcgctgac
gatgtagcatcacagcactgacttctcttcagggtccaagctggttcttt
tgctcacctgtaaaacctctctgaatgtcttgtcttgccgacggatggta
atctgtgatcacacattgtgtggcgtataggggaaagcacgggctgttca
cggtgcgttgt

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.