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GrainGenes Sequence Report: BE489573

Sequence
BE489573
Contig
NSFT03P2_Contig2870
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC320314
wEST map position
BE489573
NCBI UniGene
Ta.3233
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, weakly similar to NP_001132609.1 hypothetical protein LOC100194082 [Zea mays]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4AL
5DL
6AL
Clone Library
Wheat unstressed seedling shoot normalized cDNA library
Tissue
Etiolated shoot
Developmental Stage
Five day old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1060_B12_C24ZS Wheat unstressed seedling shoot normalized cDNA library Triticum aestivum cDNA clone WHE1060_B12_C24, mRNA sequence.
Other Name
WHE1060_B12_C24ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli DH10B
DNA Library
TA006E2N
Clone
WHE1060_B12_C24
Probe
WHE1060_B12_C24
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.3233.2.A1_a_at226WHE2AFFY
Ta.3233.1.A1_at149WHE2AFFY
DNA
gacaagtatcagttcttgcggatagatttccagctttaaagacattcaga
agctgtgacttgctgccatccagttggatgtctgttgcatggtatgtttc
aaacacttttgagcctgtttactcggtcatttttccagtggtaagatgtg
ttatgttccatttgttggctgccatgtattatcctcttttgctgtagatg
ttcctaaacttctgagagttttaattatttgtgatcaattattccgtaat
acatgccatcctcattatcctcttattacctgccatgtattatcctcgta
ttacctgccatgtattatcctcttattattatctgccatcctcattttta
tttgaaagcatctggccataacgttgtacctgtttttctgtagacaacgt
tttggtttcattataattaagcatgatgccatcacagtactggccaagtt
ataacatttgccgactgtgctggcccatgcagaggatgcctaccagttgc
catgcctaaacaactaggcatgcctatcgtgctgcatttcagcatactgt
gctgta

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