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GrainGenes Sequence Report: BE490223

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Sequence	BE490223
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene	Ta.9249
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001049340.1 Os03g0210400 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat cold-stressed seedling cDNA library
Tissue	Seedling
Developmental Stage	Five-day old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0366_G10_N20ZS Wheat cold-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0366_G10_N20, mRNA sequence.
Other Name	WHE0366_G10_N20ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA007E1X
Clone	WHE0366_G10_N20
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.9249.1.S1_at 141 WHE2AFFY Ta.9249.1.S1_at 127 WHE2AFFY
DNA	ttcatgtcaagcctcttgacaaaccagagcttgaaaattggcacagctat cttgattttatcgagagggaagaagacattaataagcttcctttgtaatg ggatattgaactgaggatagagcgtggacataatgtttggtttcgatgag gctaagtactccaaactttcatttgtcagtggccgttctgggttaatgat atttggtaccaaatatcagttgtttgaattttgaatctcatatatgcagg tatttgcttaatgctcaccttcttaaccatctatctatcgagcaaggatg gtccctcctttcttttggaggggccaacctaatcagctaatggagtaacc tgtgtgaacttctctttttgttgtcattatcattgttcttttgtgcttta tattcatttttctttcttcttcttgtctattttctgatggtacacaatac cgtttcaggttatcaagttatacgaaagatgtgttattgcgtgtgctagc tattcagaattctggatccgctatgtgcaatgcatggaggatagacaaag cct

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