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GrainGenes Sequence Report: BE492262

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Sequence
BE492262
Contig
NSFT03P2_Contig18185
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
DB Remark
Locus Source: Triticum monococcum
Keyword
EST
Germplasm
T. monococcum ssp. monococcum DV92
Species
Triticum monococcum
Cultivar
DV92
Clone Library
Triticum monococcum vegetative apex cDNA library
Tissue
Vegetative shoot apex
Developmental Stage
Three weeks-old plants
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0556_G10_G10ZE Triticum monococcum vegetative apex cDNA library Triticum monococcum cDNA clone WHE0556_G10_G10, mRNA sequence.
Other Name
WHE0556_G10_G10ZE  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli XLOLR
DNA Library
TM011XXX
Clone
WHE0556_G10_G10
Probe
[Wcl200ct818cn1242]
{SpCl-18}
BE492262
Remark
DB_xref: taxon:4568
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene T3 primer.
Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tagggtttccagttgcaagccgcgccgccgccgccgccgccatgggacac
tcgaacgtctggaactcgcaccccaagaactacggccccggatccagggt
ttgccgtgtctgcggcaactcgcatggcctgatccgcaagtaccggctga
tgtgctgcaagcagtgcttccgcagcaacgccaaggacattggcttcatc
aagtaccgttaaggatggaaacctcaaaggaagcaaggactaggaaatta
tcaacaaactgtggtgcttcatcgaggctgatctttatcctataagctat
gtaatgaactactattatgtttgaatcctggtgacaaaaccgtgaaacca
tgttagtgactgctgt

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